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Association of MMP9 Polymorphism with Myocardial Infarction in Pakistani Population

Riffat Iqbal, Aneeqa Zafar, Arslan Habib, Farhat Ijaz, Shaista Aslam, Rabbia Musaddaq, Maryam Mukhtar, Rabia Jahangir, Ghulam Jaffar, Muhammad Asif, Muhammad Shehzad, Muhammad Usman Taj, Muhammad Umair

Background: Myocardial Infarction (MI) is the most common type of coronary heart disease. The current study was conducted to identify the susceptibility of the MMP-9 gene among families affected by myocardial infarction in the Pakistani population.

Methods: A family clustering study based on 5 families having myocardial infarction patients was conducted. Blood samples from patients and their family members were collected for further genetic analysis. Patients were with a mean BMI (Body Mass Index) of (30.2 ± SD) kg/m². The mean age for diagnosis of disease was (50 ± SD) years. Genomic DNA was isolated from the blood through manual extraction. Primers were optimized and genotyping was done by Polymerase Chain Reaction (PCR) which was followed by DNA sequencing and Restriction Fragment Length Polymorphism (RFLP).

Results: As a result of polymorphism, A into G and C into T conversions were identified on rs17576 and rs3918242 polymorphic sites on the MMP-9 gene respectively.

Conclusion: In conclusion smoking, hypertension, diabetes and polymorphism of rs17576 and rs3918242 were significantly associated with the onset of MI in the Pakistani population and males were at higher risk. Further studies should be conducted on large scale to evaluate the association of MMP-9 polymorphism with MI.